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Categoric prediction of metal ion mechanisms in the active sites of 17 select type II restriction endonucleases

✍ Scribed by Sonia Advani; Puneet Mishra; Shraddha Dubey; Samridhi Thakur


Book ID
116300902
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
156 KB
Volume
402
Category
Article
ISSN
0006-291X

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The number of metal ions required for phosphoryl transfer in restriction endonucleases is still an unresolved question in molecular biology. The two Ca 2q and Mn 2q ions observed in the pre-and post-reactive complexes of BamHI conform to the classical two-metal ion choreography. We probed the Mg 2q