Metabolizable111in chelate conjugated anti-idiotype monoclonal antibody for radioimmunodetection of lymphoma in mice

✍ Scribed by Michael K. Haseman; David A. Goodwin; Claude F. Meares; Mark S. Kaminski; Theodore G. Wensel; Michael J. McCall; Ronald Levy

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 577 KB
• Volume: 12
• Category: Article
• ISSN: 0340-6997
• DOI: 10.1007/bf00254750

✦ Synopsis

The relative biological properties of 111In-labeled monoclonal antibodies (MoAb) coupled with a conventional bifunctional chelate (BC) and a new, enzyme metabolizable, bifunctional chelate (BCM) were investigated. A rat IgG2a MoAb against idiotype from a mouse B-cell lymphoma was utilized. Mice bearing B-cell lymphomas in the subcutaneous tissues of the flank were given IV-injections of labeled MoAb and imaged or killed for organ counting at 24 h or 48 h. Rat anti-dinitrophenyl IgG2a MoAb and non-specific polyclonal mouse IgG were used as controls. Compared to BC, the use of BCM resulted in a substantial decrease in blood background activity, a shorter biological half-life and an increase in tumor to blood ratio at the expense of a moderate decrease in absolute tumor uptake. The versatile chemistry of these C-1 substituted bifunctional chelates provides a variety of possible enzyme cleavable moieties for further investigation.