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Mechanisms of increasing expression of a yeast gene in Escherichia coli

✍ Scribed by Miles B. Brennan; Kevin Struhl


Book ID
118918252
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
430 KB
Volume
136
Category
Article
ISSN
0022-2836

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All five tryptophan biosynthetic genes of Saccharomyces cerevisiae were unified on plasmid pME554, which is based on 2 micrometer DNA and pBR322 sequences allowing for autonomous replication in yeast and E. coli. Homologous and heterologous expression of this artificial yeast TRP-gene cluster was st