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Differential Expression of Cloned Yeast Dihydrofolate Reductase Gene in Escherichia coli

โœ Scribed by KAMALENDU NATH; JOSEPH CANNILLO; EDWARD W. BAPTIST


Book ID
118721333
Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
115 KB
Volume
435
Category
Article
ISSN
0890-6564

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Cloning of a yeast dihydrofolate reducta
โœ Kamalendu Nath; Edward W. Baptist ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 546 KB

The dihydrofolate reductase gene of Saccharornyces cerevisiae has been isolated by selection of trimethoprim resistant Escherichia coli transformed with a gene bank of yeast DNA in plasmid pBR322. From a 9.2 kilobase pair BamHI DNA fragment this gene has been localized to a 1.76 kb fragment, the res