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Characterization of the cloned Escherichia coli dihydrofolate reductase

โœ Scribed by Julian I. Rood; Jeffrey W. Williams


Book ID
113124328
Publisher
Elsevier Science
Year
1981
Weight
347 KB
Volume
660
Category
Article
ISSN
0005-2744

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Cloning of a yeast dihydrofolate reducta
โœ Kamalendu Nath; Edward W. Baptist ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 546 KB

The dihydrofolate reductase gene of Saccharornyces cerevisiae has been isolated by selection of trimethoprim resistant Escherichia coli transformed with a gene bank of yeast DNA in plasmid pBR322. From a 9.2 kilobase pair BamHI DNA fragment this gene has been localized to a 1.76 kb fragment, the res