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Purification and properties of Escherichia coli dihydrofolate reductase

✍ Scribed by Baccanari, D.; Phillips, A.; Smith, S.; Sinski, D.; Burchall, J.


Book ID
126140015
Publisher
American Chemical Society
Year
1975
Tongue
English
Weight
832 KB
Volume
14
Category
Article
ISSN
0006-2960

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Site-specific mutagenesis of dihydrofola
✍ Jin-Tann Chen; Ruth J. Mayer; Carol A. Fierke; Stephen J. Benkovic πŸ“‚ Article πŸ“… 1985 πŸ› John Wiley and Sons 🌐 English βš– 441 KB

Two site-specific mutations of dihydrofolate reductase from Escherichiu coli based on the x-ray crystallographic structure were constructed. The first mutation (His-45 + Gln) is aimed at assessing the interaction between the imidazole moiety and the pyrophosphate backbone of NADPH. The second (Thr-1