𝔖 Bobbio Scriptorium
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Expression in yeast of an Escherichia coli gene encoding a phospholipid biosynthetic enzyme

✍ Scribed by Kelly, Beth L.; Greenberg, Miriam L.


Book ID
122909709
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
475 KB
Volume
147
Category
Article
ISSN
0378-1119

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All five tryptophan biosynthetic genes of Saccharomyces cerevisiae were unified on plasmid pME554, which is based on 2 micrometer DNA and pBR322 sequences allowing for autonomous replication in yeast and E. coli. Homologous and heterologous expression of this artificial yeast TRP-gene cluster was st