𝔖 Bobbio Scriptorium
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Yeast DNA sequences initiating gene expression in Escherichia coli

✍ Scribed by Astrid Lewin; Thi Tuyen Tran; Daniela Jacob; Martin Mayer; Barbara Freytag; Bernd Appel


Book ID
113799494
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
231 KB
Volume
159
Category
Article
ISSN
0944-5013

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All five tryptophan biosynthetic genes of Saccharomyces cerevisiae were unified on plasmid pME554, which is based on 2 micrometer DNA and pBR322 sequences allowing for autonomous replication in yeast and E. coli. Homologous and heterologous expression of this artificial yeast TRP-gene cluster was st