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Mechanism of the Idling-Turnover Reaction of the Large (Klenow) Fragment of Escherichia coli DNA Polymerase I

✍ Scribed by Valerie Mizrahi, Patricia A. Benkovic and Stephen J. Benkovic


Book ID
123632440
Publisher
National Academy of Sciences
Year
1986
Tongue
English
Weight
788 KB
Volume
83
Category
Article
ISSN
0027-8424

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The Klenow fragment of DNA polymerase I from Escherichia coli has two enzymatic activities: DNA polymerase and 3'-5' exonuclease. The crystal structure showed that the fragment is folded into two distinct domains. The smaller domain has a binding site for deoxynucleoside monophosphate and a divalent