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Mechanism of citrinin-induced dysfunction of mitochondria. II. Effect on respiration, enzyme activities, and membrane potential of liver mitochondria

✍ Scribed by Generoso M. Chagas; Ma. Bengna M. Oliveira; Annibal P. Campello; Ma. Lúcia W. Klüppel


Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
564 KB
Volume
10
Category
Article
ISSN
0263-6484

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Mechanism of citrinin-induced dysfunctio
✍ Generoso M. Chagas; Annibal P. Campello; Ma. Lúcia W. Klüppel 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 604 KB

Citrinin depresses the phosphorylation efficiency of rat renal cortical mitochondria, as inferred from the decrease of the respiratory control coefficient (RC) and ADP/O ratios. The transmembrane potential (A+) developed by energized mitochondria and the depolarization upon ADP addition are also dec

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The inhibition by citrinin (CTN) of lipid peroxidation of mitochondria, sub-mitochondrial particles (SMP) and microsomes was studied. This eect was reversed by the presence of high concentrations of Fe 3 (0 . 4 and 0 . 5 mM), suggesting chelation of the mycotoxin with iron or interference in the red

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The effects of the mycotoxin citrinin on renal cortical and liver mitochondrial swelling were studied. Citrinin decreases the rate of swelling induced by the valinomycin-K+ complex, suggesting that the mycotoxin interferes with the mitochondrial membrane fluidity. Citrinin promotes reduction of the

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The eects of citrinin in the maintenance of the homeostasis of the reactive oxygen species in rat liver cells were evaluated. Citrinin (CTN) modi®es the antioxidant enzymatic defences of cells through the inhibition of GSSGreductase and transhydrogenase. No eect was observed on GSH-peroxidase, catal

Effects of piperine on enzyme activities
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## Abstract The influence of piperine on the enzymes and bioenergetic functions in isolated rat liver mitochondria and hepatocytes was studied. Piperine at lower concentrations (<50 μM) did not affect the RCR and ADP:O ratios, state 4 and 3 respirations supported by site‐specific substrates, viz. g