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Measurement of α-keto acids in plasma using an amino acid analyzer

✍ Scribed by Simon P. Robins; Peter J. Reeds

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
358 KB
Volume
142
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for measuring keto acid concentrations in physiological fluids using an amino acid analyzer was developed. AAer preliminary deproteinization and removal of amino acids, reduction with sodium cyanoborohydride at IOYC resulted in efficient convemion of the keto acids to their corresponding amino acids. In applying the technique to plasma samples, the use of MeOH for deproteinization was necessary to avoid the large losses of keto acids that occurred during precipitation of proteins with perchloric acid. The method was used to follow plasma ketoisocaproate concentrations in rat plasma after administration of leucine, and was sufficiently sensitive to detect concomitant changes in other branched-chain keto acid concentrations.

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