𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-performance liquid chromatographic determination of α-keto acids in human urine and plasma

✍ Scribed by Tokishi Hayashi; Hironori Tsuchiya; Hidemi Todoriki; Hiroshi Naruse

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
486 KB
Volume
122
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A high-performance liquid chromatographic method has been developed for the determination of a-keto acids in human urine and plasma. These acids were prepurified using a column of hydrazide gel and derivatized with o-phenylenediamine into 2-quinoxalinol derivatives, which were extracted into ethyl acetate. The 2-quinoxialinol derivatives were separated by reversed-phase paired-ion chromatography using a 250 X 4-mm-i.d. column packed with LiChrosorb RP-8 (5 rm). This method is sensitive, selective, and reproducible. The cY-keto acids in urine and plasma from normal individuals were determined.

📜 SIMILAR VOLUMES

High-performance liquid chromatographic
High-performance liquid chromatographic determination of the enantiomers of flecainide in human plasma and urine
✍ S. Alessi-Severini; F. Jamali; F. M. Pasutto; R. T. Coutts; S. Gulamhusein 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 486 KB

A stereospecific high-performance liquid chromatographic method for the determination of (R,S)-flecainide acetate [(R,S)-N-(2-piperidylmethyl)-2,5-bis-(2,2,2-trifluoroethoxy)benzam ide acetate] in human plasma and urine is described. After addition of the internal standard [IS; (R,S)-N-(2-piperidylm