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Measurement of Trimethylamine and Trimethylamine N-Oxide Independently in Urine by Fast Atom Bombardment Mass Spectrometry

✍ Scribed by Orval A. Mamer; Luc Choinière; Eileen P. Treacy

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
86 KB
Volume
276
Category
Article
ISSN
0003-2697

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✦ Synopsis

We report a method based upon fast atom bombardment mass spectrometry (FAB-MS) and stable isotope dilution techniques for the measurement of urinary trimethylamine (TMA) and trimethylamine N-oxide (TMAOx). TMA is extracted from urine that was spiked with 15 N-labeled TMA. The extracted TMA isotopomers are quaternized with trideuteromethyl iodide and analyzed in FAB-MS with hexaethylene glycol as matrix. TMAOx is measured by evaporation of another sample of the urine spiked with 15 N-labeled TMAOx on the FAB probe and analyzed as for the TMA. The method allows the ready and simple distinguishing of controls and patients with TMAuria, and is useful in monitoring patients with the disorder. We give examples of its use in determining normal control ranges for these metabolites and in evaluating patients.

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