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A flow injection electrospray ionization tandem mass spectrometric method for the simultaneous measurement of trimethylamine and trimethylamine N-oxide in urine

✍ Scribed by David W. Johnson

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
129 KB
Volume
43
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

Key metabolites for the diagnosis of the genetic disorder trimethylaminuria are trimethylamine (TMA) and trimethylamine N‐oxide (TMAO). A rapid, automatable flow injection ESI‐MS/MS method for their measurement in urine has been developed. The TMA was derivatized with ethyl bromoacetate to form ethyl betaine bromide. The 2 min ESI‐MS/MS analysis employed four multiple reaction monitoring (MRM) ion pairs for derivatized TMA (146.1, 118.1), derivatized ^2^H~9~‐TMA (155.1, 127.1), TMAO (76.1, 58.1) and ^2^H~9~‐TMAO (85.1, 66.1). In control urine samples (n = 27) referred for suspected metabolic problems TMA was 0.11–1.19 mmol/mol creatinine, TMAO was 13.5–181 mmol/mol creatinine and the TMA/TMAO ratio was 0.0025–0.055. In five patients with diagnosed trimethylaminuria, TMA was 5.3–230 mmol/mol creatinine, TMAO was 0.36–607 mmol/mol creatinine and the TMA/TMAO ratio was 0.20–134. Copyright © 2007 John Wiley & Sons, Ltd.

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