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Measurement of caspase activity in individual cells reveals differences in the kinetics of caspase activation between cells

✍ Scribed by Morgan, M J; Thorburn, A


Book ID
110016168
Publisher
Nature Publishing Group
Year
2001
Tongue
English
Weight
422 KB
Volume
8
Category
Article
ISSN
1350-9047

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We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases. Capture on an ELISA plate with an antibody speci