Quantification of Active Caspase 3 in Ap
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Paul A. Saunders; Jeffrey A. Cooper; Michelle M. Roodell; Dorothy A. Schroeder;
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Article
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2000
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Elsevier Science
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English
⚖ 348 KB
We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases. Capture on an ELISA plate with an antibody speci