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Evaluation of caspase activity in apoptotic cells

✍ Scribed by Camilla Köhler; Sten Orrenius; Boris Zhivotovsky


Book ID
114051011
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
295 KB
Volume
265
Category
Article
ISSN
0022-1759

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We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases. Capture on an ELISA plate with an antibody speci