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Caspases Mediate Pannexin 1 Channel Activation in Apoptotic Cells

✍ Scribed by Sandilos, Joanna K.; Chekeni, Faraaz B.; Elliott, Michael R.; Walk, Scott F.; Kinchen, Jason M.; Lazarowski, Eduardo R.; Armstrong, Allison J.; Penuela, Silvia; Laird, Dale W.; Salvesen, Guy S.; Isakson, Brant E.; Ravichandran, Kodi S.; Bayliss, Douglas A.


Book ID
119208513
Publisher
Biophysical Society
Year
2011
Tongue
English
Weight
36 KB
Volume
100
Category
Article
ISSN
0006-3495

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We describe an enzyme-linked immunosorbent assay (ELISA) for quantifying relative amounts of active caspase 3 in apoptotic cells. Covalent modification of caspase 3 active sites with a biotinylated inhibitor differentiates active from latent caspases. Capture on an ELISA plate with an antibody speci