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Mass Spectrometric Determination of O-Glycosylation Sites Using β-Elimination and Partial Acid Hydrolysis

✍ Scribed by Mirgorodskaya, Ekaterina; Hassan, Helle; Clausen, Henrik; Roepstorff, Peter


Book ID
121375378
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
118 KB
Volume
73
Category
Article
ISSN
0003-2700

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A sensitive mass spectrometric method for the specific identification of 0-glycosylation sites in glycopeptides is presented. Reductive j-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determin