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Partial Vapor-Phase Hydrolysis of Peptide Bonds: A Method for Mass Spectrometric Determination of O-Glycosylated Sites in Glycopeptides

โœ Scribed by Ekaterina Mirgorodskaya; Helle Hassan; Hans H. Wandall; Henrik Clausen; Peter Roepstorff

Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
194 KB
Volume
269
Category
Article
ISSN
0003-2697

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A sensitive mass spectrometric method for the specific identification of 0-glycosylation sites in glycopeptides is presented. Reductive j-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determin