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Determination of glycosylation sites in O-linked glycopeptides: A sensitive mass spectrometric protocol

✍ Scribed by Geert Jan Rademaker; Johan Haverkamp; Jane Thomas-Oates

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 458 KB
Volume: 28
Category: Article
ISSN: 1076-5174
DOI: 10.1002/oms.1210281228

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✦ Synopsis

A sensitive mass spectrometric method for the specific identification of 0-glycosylation sites in glycopeptides is presented. Reductive j-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determine the sequence of the modified peptide, in which the position of the modified amino acid marks the site of 0-glycosylation. The method also provides information on the carbohydrate chain and is applicable at the low nanomolar level.

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