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Mass Spectrometric Determination of the Sites of O-Glycan Attachment with Low Picomolar Sensitivity

โœ Scribed by Geert Jan Rademaker; Spiros A. Pergantis; Leonore Blok-Tip; James I. Langridge; Astrid Kleen; Jane E. Thomas-Oates

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
223 KB
Volume
257
Category
Article
ISSN
0003-2697

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Determination of glycosylation sites in
Determination of glycosylation sites in O-linked glycopeptides: A sensitive mass spectrometric protocol
โœ Geert Jan Rademaker; Johan Haverkamp; Jane Thomas-Oates ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 458 KB

A sensitive mass spectrometric method for the specific identification of 0-glycosylation sites in glycopeptides is presented. Reductive j-elimination liberates a peptide containing a specifically modified amino acid at the former site of carbohydrate attachment. Mass spectrometry is used to determin