Mapping of ornithine aminotransferase gene sequences to mouse Chromosomes 7, X, and 3

✍ Scribed by Vijaya Ramesh; Shirley V. Cheng; Christine A. Kozak; Bruce J. Herron; Vivian E. Shih; Benjamin A. Taylor; James F. Gusella

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 584 KB
• Volume: 3
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00355836

✦ Synopsis

Ornithine aminotransferase (OAT), a mitochondrial matrix enzyme, is deficient in patients with gyrate atrophy of the choroid and retina. In human, the OAT structural gene maps to Chromosome (Chr) 10q26 and several OAT-related sequences, some of which are known to be processed pseudogenes, which map to Xp11.3-11.21. Here, we report chromosomal localization in the mouse of the OAT gene and related sequences. Genomic DNA blot analysis of a well-characterized panel of Chinese hamster x mouse somatic cell hybrids using a human OAT probe revealed two murine loci, one on mouse Chr 7 and the other on Chr X. In addition, segregation of restriction fragment length polymorphisms (RFLPs) detected by the OAT probe in recombinant inbred (RI) strains detected a third locus on Chr 3 and positioned the X locus near Cf-8 and Rsvp. Progeny of an intersubspecific backcross were used to map the Chr 7 locus between Tyr and Int-2, near Cyp2e-1.