Genetic mapping of DNA topoisomerase I-related sequences to mouse Chromosomes 2 and 14

✍ Scribed by S. F. Kingsmore; C.-K. Lo; J. Hwang; C.-F. Hui; M. F. Seldin

• Publisher: Springer-Verlag
• Year: 1994
• Tongue: English
• Weight: 289 KB
• Volume: 5
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00360556

✦ Synopsis

DNA topoisomerase I is a highly conserved and ubiquitously expressed protein of eukaryotes. D N A topoisomerase I is indispensable in DNA transcription and replication and in the maintenance of genomic stability, relaxing D N A s u p e r c o i l s , and s u p p r e s s i n g c h r o m o s o m a l recombination (Wang 1991). In human, the gene encoding D N A topoisomerase I (TOP1) has been identified and localized to human Chromosome (Chr) 20 ( D ' A r p a et al. 1988;Juan et al. 1988;Kunze et al. 1989). The present report describes genetic mapping of two mouse topoisomerase I-related sequences (Top-1 and Top-lrs).

The chromosomal location of Top-1 in the mouse was determined with a panel of DNA samples from an interspecific cross that has been characterized for over 600 genetic markers throughout the genome. RFLVs were determ i n e d by S o u t h e r n blot h y b r i d i z a t i o n of D N A from C3H/HeJ-gId and (C3H/HeJ-gld • Mus spretus)F I parental