Mapping of chromosomal gains and losses in primitive neuroectodermal tumors by comparative genomic hybridization

✍ Scribed by Barbara R. Schütz; Wolfram Scheurlen; Jürgen Krauss; Stanislas du Manoir; Stefan Joos; Martin Bentz; Peter Lichter

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 633 KB
• Volume: 16
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199607)16:3<196::aid-gcc7>3.0.co;2-u

✦ Synopsis

A series of 18 primitive neuroectodermal tumors (PNETs), the most common malignant central nervous system tumors of childhood. were analyzed with the recently developed approach of comparative genomic hybridization (CGH). In five cases, in which only small amounts of DNA were available, universal polymerase chain reaction was successfully applied to generate adequate probe material. In I 5 tumors, chromosomal imbalances were elicited, most frequently involving chromosome I 7 (loss of I7p and gain of I7q). Further recurrent imbalances included gains of the distal regions of 4p. 5p. 5q, 7q, 8q, and 9p. High-level amplifications were found on 2p24 (one case) and 8q24 (three cases), suggesting involvement of the protooncogenes MYCN and MYC. respectively. In one of these cases, Southern blot analysis could be performed, proving high-copy-number amplification of MYC. Interestingly, none of the three patients with high-copy-number amplifications of MYC responded to therapy.