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Detection of chromosomal gains and losses in comparative genomic hybridization analysis based on standard reference intervals

✍ Scribed by Maria Kirchhoff; Tommy Gerdes; Hanne Rose; Jan Maahr; Anne Marie Ottesen; Claes Lundsteen

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 487 KB
Volume: 31
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19980301)31:3<163::aid-cyto3>3.0.co;2-m

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✦ Synopsis

Criteria for detection of chromosome aberrations by Comparative Genomic Hybridization (CGH) are not standardized and improvement of this part of the analysis is of paramount importance to the applicability of the technique. The aim of this work was to suggest CGH detection criteria that increase the specificity and sensitivity and at the same time include chromosome regions previously excluded from CGH analysis. We analyzed 33 hybridizations with normal DNA and modified our CGH software in order to use a selection of these normal analyses as a model for interpretation of analyses of unknown samples. This approach was successfully tested on 14 samples with known aberrations.

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