Gains and losses of DNA sequences in liposarcomas evaluated by comparative genomic hybridization

✍ Scribed by Jadwiga Szymanska; Maija Tarkkanen; Tom Wiklund; Martti Virolainen; Carl Blomqvist; Sirpa Asko-Seljavaara; Erkki Tukiainen; Inkeri Elomaa; Sakari Knuutila

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 452 KB
• Volume: 15
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199602)15:2<89::aid-gcc2>3.0.co;2-#

✦ Synopsis

Comparative genomic hybridization (CGH) was used to detect and map the regions of gain, high-level amplification, and loss of DNA sequences in 14 liposarcomas. Thirteen tumors showed DNA sequence copy number changes of one or more genomic regions (mean, six aberrationdtumor; range, 0-1 7). These aberrations were observed in almost every chromosome but some chromosomal regions were affected more often than others. DNA sequence gains were more frequent than losses. The most common gain was seen at I2q 14-2 I (50% of tumors). Other frequent gains (29%) were of I q2 I-24,8cen-q2 I .2, I9q, and 20q. High-level amplification was observed in six (43%) tumors and included as minimal common segments bands 129 15, I q22, and I q24. In five (36%) tumors, sequences at I q2 1-24 and I q32 were found to be gained simultaneously with 12q 14-21, which means that in 7 I % of the tumors with gain at I 2q, an increase of DNA sequence copy number at I q was also observed. The most common losses of DNA sequences (2 I %) occurred from regions 9p2 I -pter and I3q2 I -qter. Most of the aforementioned regions have not previously been reported t o be altered in liposarcomas. The detection of a novel recurring amplicon at I q21-24 with high-level amplification at I q22 and frequent simultaneous DNA sequence gain at I 2q 14-21 (high-level amplification at I29 15) suggests that genes linked t o both these regions may play a significant role in the development and progression of liposarcomas.