Comparative genomic hybridization reveals a specific pattern of chromosomal gains and losses during the genesis of colorectal tumors

✍ Scribed by Thomas Ried; Regina Knutzen; Rüdiger Steinbeck; Harald Blegen; Evelin Schröck; Kerstin Heselmeyer; Stanislas du Manoir; Gert Auer

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 848 KB
• Volume: 15
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/(sici)1098-2264(199604)15:4<234::aid-gcc5>3.0.co;2-2

✦ Synopsis

Comparative genomic hybridization was used to screen the DNA extracted from histologically defined tissue sections from consecutive stages of colorectal carcinogenesis for chromosomal aberrations. N o aberrations were detected in normal epithelium (n = 14). Gain of chromosome 7 occurred as a single event in low-grade adenomas (n = 14). In high-grade adenomas (n = 12), an overrepresentation of chromosomes 7 and 20 was present in 30% of the cases analyzed. The transition t o colon carcinomas (n = 16) was characterized by the emergence of multiple chromosomal aberrations. Chromosomes I, I 3, and 20 and chromosome arms 7p and 8q were frequently gained, whereas chromosome 4 and chromosome arms 8p and 18q were recurrently underrepresented. The same tissue sections that were used for CGH were analyzed by means of DNA-ploidy measurements and immunohistochemical staining to quantify proliferative activity and p2 I W A F -I and TP53 expression. We observed that crude aneuploidy and increased proliferative activity are early events in colorectal carcinogenesis, followed by TP53 overexpression and the acquisition of recurrent chromosomal gains and losses during the progression from high-grade adenomas to invasive carcinomas. Genes Chrornosorn Cancer 15234-245 ( I 996).