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Loss of heterozygosity analysis in malignant gliomas

✍ Scribed by Masahiro Mizoguchi; Daisuke Kuga; Yanlei Guan; Nobuhiro Hata; Akira Nakamizo; Koji Yoshimoto; Tomio Sasaki


Publisher
Springer Japan
Year
2011
Tongue
English
Weight
317 KB
Volume
28
Category
Article
ISSN
1433-7398

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Cytogenetic and RFLP studies have shown that chromosome I0 is frequently lost in tumor cells from glioblastomas, suggesting that a suppressor gene important in tumorigenesis is present on this chromosome. Forty-one tumors were examined for loss of heterozygosity at 23 loci on chromosome 10 to determ

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Loss of constitutional heterozygosity as determined through the analysis of restriction-fragment-length polymorphism (RFLP) on tumoral and constitutional DNA has proven to be helpful to delimit the location of tumor-suppressor genes in the human genome. In malignant gliomas this approach indicates t