Localization of the gene for human heart fatty acid binding protein to chromosome 1p32-1p33

✍ Scribed by Robert F. Troxler; Gwynneth D. Offner; Jen-Wei Jiang; Bai-Lin Wu; James C. Skare; Aubrey Milunsky; Herman E. Wyandt

• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 723 KB
• Volume: 92
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00420939

✦ Synopsis

Heart fatty acid binding protein (hFABP) is an abundant 14-kDa cytosolic protein thought to be involved in trafficking of fatty acids from the plasma membrane to sites of r-oxidation in mitochondria and peroxisomes and to the endoplasmic reticulum for lipid synthesis. A human hFABP cDNA isolated by polymerase chain reaction was used as a probe for in situ hybridization to metaphase chromosomes. A fragment of the gene for human hFABP was used as a probe for fluorescence in situ hybridization to metaphase chromosomes. The cDNA and genomic probes both localized the gene for human hFABP to chromosome 1p32-1p33. ganellar membranes in cardiac myocytes due to the detergent effects of fatty acids present at elevated levels in ischemia (Knowlton et al. 1989b).

In situ hybridization studies have localized the gene for human liver FABP to 2pll and the gene for human intestine FABP to 4q28-q31 (Sweetser et al. 1987). Analysis of somatic cell hybrids localized the gene for human muscle FABP to lq32-1pter (Peeters et al. 1991). The occurrence of FABP genes on different chromosomes is consistent with the early appearance of these proteins in vertebrate evolution (Chan et al. 1985). The chromosomal location of the gene for human hFABP is not known.