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Confirmation of the localization of the human recombination activating gene 1 (RAG1) to chromosome 11p13

✍ Scribed by Klaus Schwarz; Horst Hameister; Manfred Gessler; Karl-Heinz Grzeschik; Thomas E. Hansen-Hagge; Claus R. Bartram

Publisher: Springer
Year: 1994
Tongue: English
Weight: 296 KB
Volume: 93
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00210617

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✦ Synopsis

The human recombination activating gene 1 (RAG1) has previously been mapped to chromosomes 14q and 11p. Here we confirm the chromosome 11 assignment by two independent approaches: autoradiographic and fluorescence in situ hybridization to metaphase spreads and analysis of human-hamster somatic cell hybrid DNA by the polymerase chain reaction (PCR) and Southern blotting. Our results unequivocally localize RAG1 to 11p13.

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