A new one-step method for the preparation of polyacryhunide gels containing tritium and carbon-14 for liquid scintillation counting is presented. Forty-four hours after placing the gel slices in the scintillation fluid described in the text, the samples are ready for counting. This one-step method i
Liquid scintillation counting of 3H and 32P RNA in slices of polyacrylamide gels
β Scribed by Per Hellung-Larsen
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 457 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0003-2697
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Large (200 mm3) slices of polyacrylamide gels crosslinked with N,N'-diallyltartardiamide which contain tritium-labeled protein are readily solubilized in periodic acid for liquid scintillation counting of radioactivity, but the apparent recovery of label never exceeds 82%. Extraction of the slices w
Tritium (3H)-labeled material within polyacrylamide gel slices is commonly quantified by four general liquid scintillation counting methods: combustion, gel solubilization, selective solubilization of modified crosslinked gels, and elution. Of these four methods examined in this study, only combusti
A method is described for analyzing the radioactivity of 3H-labeled RNA after separation by gel electrophoresis. The gels were soaked in 10% acetic acid and were sliced. The gel slices were dehydrated in alcohol, then saturated with toluene, and finally permeated with a toluene-based scintillation f