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Liquid scintillation counting of polyacrylamide gels crosslinked with N,N′-methylene-bis-acrylamide and N,N′-diallyltartardiamide

✍ Scribed by Paul A. Moore

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
419 KB
Volume
108
Category
Article
ISSN
0003-2697

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✦ Synopsis

Tritium (3H)-labeled material within polyacrylamide gel slices is commonly quantified by four general liquid scintillation counting methods: combustion, gel solubilization, selective solubilization of modified crosslinked gels, and elution. Of these four methods examined in this study, only combustion ensured complete recovery of 3H label; however, a less expensive and more convenient elution method yielding both a high recovery and cocktail counting efficiency was the use of Soluene-350 with 0.55% Permablend III in toluene. This particular solubilizer-cocktail system eliminates almost all chemiluminescence as does combustion.

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✍ Benny K. George; V.N. Rajasekharan Pillar 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 299 KB

Copolymers of acrylamide and N,N'-methylene-bis-acrylamide were prepared by free radical solution polymerization. The resulting crosslinked polyacrylamide gels containing 5, 10, 15 and 20% of the crosslinking agent were converted to the corresponding N-bromo derivatives by treatment with potassium h