Partial purification and some properties
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B. Bisakowski; S. Kermasha; C. Schuepp
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Article
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1995
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Springer
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English
โ 236 KB
A partially purified lipoxygenase extract was obtained from the yeast Saccharomyces cerevisiae by precipitation with solid (NH4)SO4 at 20% to 80% saturation. The enzyme had two pH optima, at pH 8.0 and 10.0, with respective apparent K m values of 13 and 9.5 ฮผM. At both pH optima, the lipoxygenase de