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Limitations of the Usefulness of the Limulus Assay for Endotoxin

โœ Scribed by Stumacher, Russell J.; Kovnat, Marilyn J.; McCabe, William R.

Book ID
126815780
Publisher
Massachusetts Medical Society
Year
1973
Tongue
English
Weight
683 KB
Volume
288
Category
Article
ISSN
0096-6762

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๐Ÿ“œ SIMILAR VOLUMES

Improved microtechnique for endotoxin as
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โœ Andreas Gardi; Gian R. Arpagaus ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 292 KB

MATERIALS AND METHODS ## Reagents. Lyophilized Litnultrs amebocyte lysate (Pyrogent. purchased from Mallinckrodt (St. Louis, MO.) was used in all experiments. The redissolved reagent was kept frozen at -20ยฐC in small aliquots for no longer than 4 weeks.

Quantitative Limulus lysate assay for en
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This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentr