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Improved microtechnique for endotoxin assay by the Limulus amebocyte lysate test

✍ Scribed by Andreas Gardi; Gian R. Arpagaus


Book ID
102627846
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
292 KB
Volume
109
Category
Article
ISSN
0003-2697

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✦ Synopsis


MATERIALS AND METHODS

Reagents. Lyophilized

Litnultrs amebocyte lysate (Pyrogent.

purchased from Mallinckrodt (St. Louis, MO.) was used in all experiments.

The redissolved reagent was kept frozen at -20Β°C in small aliquots for no longer than 4 weeks.


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Specific assay for endotoxin using immob
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The LAL test is inhibited or enhanced by many substances. To overcome these problems, we have developed a specific endotoxin assay method using an ultrafiltration unit, a fluorometric LAL reagent, and immobilized histidine (which is a specific adsorbent for endotoxins). This method is composed of tw