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The "Limulus" Amebocyte Lysate Test for Endotoxin

✍ Scribed by Frederick C. Pearson III and Marlys Weary


Book ID
124447181
Publisher
University of California Press
Year
1980
Tongue
English
Weight
317 KB
Volume
30
Category
Article
ISSN
0006-3568

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MATERIALS AND METHODS ## Reagents. Lyophilized Litnultrs amebocyte lysate (Pyrogent. purchased from Mallinckrodt (St. Louis, MO.) was used in all experiments. The redissolved reagent was kept frozen at -20Β°C in small aliquots for no longer than 4 weeks.

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The LAL test is inhibited or enhanced by many substances. To overcome these problems, we have developed a specific endotoxin assay method using an ultrafiltration unit, a fluorometric LAL reagent, and immobilized histidine (which is a specific adsorbent for endotoxins). This method is composed of tw