Limulus amebocyte lysate test for endotoxemia
โ Scribed by Fink, P. C. ;Lehr, L. ;Urbaschek, R. M. ;Kozak, J.
- Book ID
- 105211123
- Publisher
- Springer-Verlag
- Year
- 1981
- Tongue
- English
- Weight
- 732 KB
- Volume
- 59
- Category
- Article
- ISSN
- 1432-1440
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
MATERIALS AND METHODS ## Reagents. Lyophilized Litnultrs amebocyte lysate (Pyrogent. purchased from Mallinckrodt (St. Louis, MO.) was used in all experiments. The redissolved reagent was kept frozen at -20ยฐC in small aliquots for no longer than 4 weeks.
The LAL test is inhibited or enhanced by many substances. To overcome these problems, we have developed a specific endotoxin assay method using an ultrafiltration unit, a fluorometric LAL reagent, and immobilized histidine (which is a specific adsorbent for endotoxins). This method is composed of tw