✦ LIBER ✦

Quantitative Limulus lysate assay for endotoxin activity: Aggregation of radioiodinated coagulogen monomers

✍ Scribed by Robert S. Munford

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 354 KB
Volume: 91
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90537-7

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✦ Synopsis

This communication describes a modification of the Limulus lysate assay which allows precise quantitation of picograms of bacterial lipopolysaccharide activity. The method measures the incorporation of 125I-labeled coagulogen monomers into the lysate clot as a function of lipopolysaccharide concentration. The method is more precise and requires less lysate than the previously described quantitative assays for endotoxin activity.