## Abstract A reversed‐phase HPLC assay method was developed to determine levels of the drugs trimethoprim, sulfamethoxazole and the N~4~‐acetyl metabolite of the latter in human blood plasma and urine. The organic substances are extracted with ethyl acetate by a single extraction at pH 6.8. The me
Laboratory Testing of Sulfamethoxazole and its Metabolite Acetyl-Sulfamethoxazole in Soil
✍ Scribed by Sibylla Höltge; Robert Kreuzig
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 590 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1863-0650
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## Abstract Sulfamethoxazole was labelled at positions 3, 5, and 4′ by H/D exchange in a mixture of 5% ^2^H~2~SO~4~ and 95% ^2^H~2~O (v/v) under reflux for 72h with good isotope incorporation and acceptable yield. Subsequently, [^2^H~3~]‐sulfamethoxazole‐__N__~1~‐glucuronide and [^2^H~3~]‐__N__~4~‐
A HPLC method for the determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma is reported. The metabolites under investigation were the N4-acetyl sulfonamides and 3'- and 4'-demethyl trimethoprim. In order to obtain a sensitivity of 25-50 ng ml-
A complete analytical procedure, including sample clean-up and a micellar electrokinetic chromatographic method, is presented for the determination of sulfamethoxazole, trimethoprim, and their main metabolites by using 20 mmol L(-1) borate buffer (pH 9.3), 25 mmol L(-1) sodium dodecylsulfate, and 5%