Determination of Sulfamethoxazole and Trimethoprim and Their Metabolites in Hospital Effluent
β Scribed by Carla G. B. Brenner; Carlos A. Mallmann; Daniel R. Arsand; Francieli M. Mayer; Ayrton F. Martins
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 430 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1863-0650
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## Abstract A capillary electrophoretic method for the simultaneous determination of sulfamethoxazole and trimethoprim in plasma was developed. Sulfamethoxazole and trimethoprim extracted from human plasma with ethyl acetate were analyzed at 20 kV and 25Β°C using 15 mm phosphate buffer (pH 6.2) as t
## Abstract A reversedβphase HPLC assay method was developed to determine levels of the drugs trimethoprim, sulfamethoxazole and the N~4~βacetyl metabolite of the latter in human blood plasma and urine. The organic substances are extracted with ethyl acetate by a single extraction at pH 6.8. The me