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Determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma by column switching HPLC

✍ Scribed by M.J.B. Mengelers; M.B.M. Oorsprong; H.A. Kuiper; M.M.L. Aerts; E.R. Van Gogh; A.S.J.P.A.M. Van Miert

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
831 KB
Volume
7
Category
Article
ISSN
0731-7085

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✦ Synopsis

A HPLC method for the determination of sulfadimethoxine, sulfamethoxazole, trimethoprim and their main metabolites in porcine plasma is reported. The metabolites under investigation were the N4-acetyl sulfonamides and 3'- and 4'-demethyl trimethoprim. In order to obtain a sensitivity of 25-50 ng ml-1, the application of column switching HPLC was investigated. An on-line preconcentration of the drugs and metabolites was preceded by an off-line sample pre-treatment. Parent compounds and metabolites were separated by reversed-phase HPLC followed by UV-detection. The mean recoveries for 4'-demethyl trimethoprim were greater than 80% while the mean recoveries for the other compounds were greater than 90%. Application of the method for analysis of plasma samples obtained from pharmacokinetic studies is described.

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