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Determination of cyclosporin A in blood and plasma by column switching-HPLC after rapid sample preparation

✍ Scribed by Nussbaumer, K. ;Niederberger, W. ;Keller, H. P.

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
353 KB
Volume
5
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

A method for the determination of cyclosporin A in human whole blood and plasma is described which uses liquid chromatography with step gradient elution and a column switching technique. The chromatographic conditions chosen allow simple and rapid sample preparation, so that a result can be obtained within one hour. Blood and plasma are deproteinized with diluted methanol and an aliquot of the clear supernatant is directly injected. The detection limit for cyclosporin A is about 20 ng/ml starting from a 0.5 ml sample. The method is sensitive enough for monitoring the drug in the therapeutic range.

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