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Labeling of steroids by activated tritium

✍ Scribed by G. Z. Tang; C. T. Peng

Publisher: John Wiley and Sons
Year: 1988
Tongue: French
Weight: 500 KB
Volume: 25
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.2580250603

No coin nor oath required. For personal study only.

✦ Synopsis

n i v e r s i t y of C a l i f o r n i a , San Francisco, CA 94143-0446 SIPQldpY S t e r o i d hormones and d e r i v a t i v e s are l a b e l e d t o high s p e c i f i c a c t i v i t i e s with a c t i v a t e d tritium generated by microwave d i s c h a r g e of tritium gas. The s t e r o i d nucleus is not degraded by r e a c t i o n s with t r i t i u m ; t h e hydroxyl and 0x0 groups a t 3, 11, 17, and 20 p o s i t i o n s of t h e s t e r o i d nucleus are not d i s p l a c e d by tritium but are oxidized or reduced t o form t h e l a b e l e d by-products. S a t u r a t i o n of A r i n g occurs r e a d i l y , but tritium a d d i t i o n t o t h e i s o l a t e d C=C double bond o c c u r s i n t h e o r d e r of A1 > As > A5.

a d d i t i o n r e a c t i o n , t h e f i n a l products from t h e u n s a t u r a t e d s t e r o i d s may s t i l l c o n t a i n s i g n i f i c a n t amounts of t h e l a b e l e d p a r e n t s .

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