l-2,4-Diaminobutyric acid as a standard in the estimation of lysine in maize samples

✍ Scribed by C. Dennison

• Publisher: Elsevier Science
• Year: 1971
• Tongue: English
• Weight: 183 KB
• Volume: 63
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)85661-2

✦ Synopsis

CHROX 5633 L-2,4=Diaminobutyric acid as a standard in the estimation of lysine in maize samples Part of a maize-breeding program required the estimation of lysine in the maize samples by automated amino acid analysis, following acid hydrolysis. Such programs require the screening of a large number of samples, making it necessary as far as possible to expedite both the preparation of samples and the subsequent chromatography stages.

As in this case it is the absolute amount of a single amino acid which is required, rather than the molar ratios of the amino acids, time-consuming, quantitative techniques would normally be necessary in the steps following acid hydrolysis. A more expeditious procedure, coupled with a gain in accuracy, can be achieved, however, by the use of an internal standard. Contribut'ons to the overall error, estimated by the use of an internal standard, are due to: (1) mechanical losses after acid hydrolysis;