Kinetics of deactivation for thermostable DNA polymerase enzymes
β Scribed by Satish C. Mohapatra; James T. Hsu
- Publisher
- Springer-Verlag
- Year
- 1996
- Tongue
- English
- Weight
- 225 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0951-208X
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π SIMILAR VOLUMES
## Abstract **A novel screening approach** based on an oligonucleotideβaddressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a libra
Heparin was found to be a potent inhibitor of a DNA polymerase present in the murine myeloma tumor MOPC-21. By increasing the KCI concentration of the reaction mixture, the inhibition of this enzyme could be completely reversed, suggesting that insoluble heparin might be a useful tool in the isolati