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Taking Fingerprints of DNA Polymerases: Multiplex Enzyme Profiling on DNA Arrays

✍ Scribed by Ramon Kranaster; Andreas Marx

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 552 KB
Volume: 48
Category: Article
ISSN: 0044-8249
DOI: 10.1002/anie.200900953

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✦ Synopsis

Abstract

A novel screening approach based on an oligonucleotide‐addressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a library of protein mutants.magnified image

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