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DNA polymerases: Perfect enzymes for an imperfect world

โœ Scribed by Anthony J. Berdis

Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
239 KB
Volume
1804
Category
Article
ISSN
1570-9639

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Use of insoluble heparin for isolation o
Use of insoluble heparin for isolation of DNA polymerase enzymes from murine myeloma
โœ Barbara A. Brennessel; D.Paul Buhrer; A.Arthur Gottlier ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 406 KB

Heparin was found to be a potent inhibitor of a DNA polymerase present in the murine myeloma tumor MOPC-21. By increasing the KCI concentration of the reaction mixture, the inhibition of this enzyme could be completely reversed, suggesting that insoluble heparin might be a useful tool in the isolati