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Use of insoluble heparin for isolation of DNA polymerase enzymes from murine myeloma

✍ Scribed by Barbara A. Brennessel; D.Paul Buhrer; A.Arthur Gottlier

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
406 KB
Volume
87
Category
Article
ISSN
0003-2697

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✦ Synopsis

Heparin was found to be a potent inhibitor of a DNA polymerase present in the murine myeloma tumor MOPC-21. By increasing the KCI concentration of the reaction mixture, the inhibition of this enzyme could be completely reversed, suggesting that insoluble heparin might be a useful tool in the isolation of DNA polymerases. When heparin covalently bound to Sepharose was used, some of the DNA polymerases present in MOPC-21 myeloma tumors were separated and partially purified.

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The possibility has been raised that either a human herpesvirus-8 (HHV-8) variant or a novel, unidentified, gamma-herpesvirus related to HHV-8 is frequently associated with multiple myeloma (MM), which could explain the lack of antibodies to HHV-8 antigens and the discordant results from polymerase