Kinetic study on thermal denaturation of hen egg-white lysozyme involving precipitation
β Scribed by Daisuke Nohara; Akihiko Mizutani; Tomoya Sakai
- Book ID
- 114399962
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 775 KB
- Volume
- 87
- Category
- Article
- ISSN
- 1389-1723
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## Abstract Based on threeβstate renaturation process of denatured proteins, an equation describing the effect of denaturant concentration on renaturation yield of denatured proteins was presented. By this equation, two parameters __n__(__m__~1~β__m__~2~) and __K__~a~ can be obtained. The former in
## Abstract Solvent isotope effects have been observed on the in vitro refolding kinetics of a protein, hen lysozyme. The rates of two distinct phases of refolding resolved by intrinsic fluorescence have been found to be altered, to differing extents, in D~2~O compared with H~2~O, and experiments h