Kinetic study of hybridoma metabolism and antibody production in continuous culture using serum-free medium
✍ Scribed by Hideo Kurokawa; Tatsuya Ogawa; Masamichi Kamihira; Yong Soo Park; Shinji Iijima; Takeshi Kobayashi
- Book ID
- 113396993
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 625 KB
- Volume
- 76
- Category
- Article
- ISSN
- 0922-338X
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## Abstract The steady‐state metabolic parameters for a hybridoma cell line have been determined in continuous suspension–perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at h
A detailed study of cell growth and antibody production kinetics in continuous culture found that the specific rate of antibody production reached a maximum saturated profile at a specific growth rate less than the maximum. This observation is novel and of importance in the understanding of the mech
A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2-10°/0 FCS), giving K,,, = 1.6 v/v% a